兽用中药玉屏风口服液质量标准提升研究
Study on Improvement of Quality Standard of Yupingfeng Oral Liquid
冯亚文 1张秋娜 2吴兰 1陈静 3张娜 3李会平 3张培训 3安莎莎 2孔红君3
作者信息
- 1. 河北省兽药饲料工作总站,石家庄 050000
- 2. 保定冀中生物科技有限公司,河北 保定 071000
- 3. 保定冀中药业有限公司,河北 保定 071500;河北省中兽药技术创新中心,河北 保定 071500
- 折叠
摘要
建立一种可同时测定玉屏风口服液中升麻素苷、5-O-甲基维斯阿米醇苷、毛蕊异黄酮葡萄糖苷含量的高效液相色谱法(HPLC).采用以十八烷基硅烷键合硅胶为填充剂的色谱柱(5μm 4.6×250 mm),以乙腈和0.2%磷酸溶液为流动相进行梯度洗脱,流速1.0mL·min-1,柱温30℃,检测波长254 nm,进样体积10 μL.检测的三种成分在测定的质量浓度范围内线性关系良好,r值均为0.999,平均加样回收率均在96%～102%之间,相对标准偏差均小于 2.0%.试验精密度、重复性、准确性和稳定性良好.新建立的检测方法简单、准确、高效,为进一步提升玉屏风口服液的质量控制提供了依据.
Abstract
To establish a high performance liquid chromatography(HPLC)method for the simultaneous determination of glucoside,5-O-methyl Visamitol side and calynoisoflavone glucoside in Yupingfeng oral liquid.The chromatography was performed on a column(5 μm 4.6×250 mm)with acetonitrile and 0.2%phosphoric acid as the fillers.The flow rate was 1.0 mL·min-1.The column temperature was 30℃.The detection wavelength was 254 nm and the sample volume was 10 μg.The linear relationship of the three components was good in the range of mass concentration,r values were 0.999,the average recoveries were between 96%and 102%,and the relative standard deviations were less than 2.0%.The test has good precision,repeatability,accuracy and stability.The newly established detection method is simple,accurate and efficient,which can provide a basis for the quality control of Yupingfeng oral liquid.
关键词
高效液相色谱/玉屏风口服液/升麻素苷/5-O-甲基维斯阿米醇苷/毛蕊异黄酮葡萄糖苷Key words
HPLC/Yupingfeng oral liquid/Glucoside/5-O-methylvisamitol glycoside/Calycosin isoflavones glucoside引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据