Study on Improvement of Quality Standard of Yupingfeng Oral Liquid
To establish a high performance liquid chromatography(HPLC)method for the simultaneous determination of glucoside,5-O-methyl Visamitol side and calynoisoflavone glucoside in Yupingfeng oral liquid.The chromatography was performed on a column(5 μm 4.6×250 mm)with acetonitrile and 0.2%phosphoric acid as the fillers.The flow rate was 1.0 mL·min-1.The column temperature was 30℃.The detection wavelength was 254 nm and the sample volume was 10 μg.The linear relationship of the three components was good in the range of mass concentration,r values were 0.999,the average recoveries were between 96%and 102%,and the relative standard deviations were less than 2.0%.The test has good precision,repeatability,accuracy and stability.The newly established detection method is simple,accurate and efficient,which can provide a basis for the quality control of Yupingfeng oral liquid.
NETL
NSTL
万方数据
维普
