从一只临床表现为流脓涕、打喷嚏的猫身上分离出一株猫疱疹病毒Ⅰ型(Feline herpesvirus type Ⅰ,FHV-1),使用F81细胞进行分离,并通过PCR、透射电镜和糖蛋白D(gD)基因序列分析来鉴定病毒.通过PCR方法从猫的鼻分泌物中扩增出1125 bp的gD基因.序列分析表明,该分离株与从GenBank获得的其他FHV-1毒株属于同一家族.随后在接种猫鼻分泌物的F81细胞的培养上清中观察到有包膜和直径约120 nm左右的疱疹样病毒颗粒.将分离获得的FHV-1病毒感染2～3月龄蓝猫,成功建立动物实验模型,感染后的猫出现脓性的眼鼻分泌物,打喷嚏等临床症状,且一周内持续排毒.该分离株为FHV-1的病原学研究提供了重要的数据,也为猫鼻气管炎疫苗候选株的筛选和疫苗的研发奠定了基础.
Isolation and Identification of a Strain of Feline Herpesvirus Type Ⅰ(FHV-1)and Establishment of an Animal Infection Model
A strain of feline herpesvirus type Ⅰ was isolated from a cat with clinical manifestations of purulent nasal discharge and sneezing.F81 cells were used for isolation,and the virus was identified by PCR,transmission electron microscopy,and glycoprotein D(gD)gene sequence analysis.A 1125 bp gD gene was amplified from nasal secretions of cats by PCR.Sequence analysis indicated that this isolate belonged to the same family as other FHV-1 strains obtained from GenBank.Subsequently,herpes-like viruses with envelopes and a diameter of about 120 nm were observed in the culture supernatant of F81 cells inoculated with cat nasal secretions.The isolated FHV-1 virus was used to infect blue cats aged 2 to 3 months,and an animal infection model was successfully established.The infected cats had purulent eye and nose secretions,sneezing and other clinical symptoms,and continued to shed the virus within a week.This isolate provides important data for the pathogenic research of FHV-1,and also lays the foundation for the screening of feline rhinotracheitis vaccine candidate strains and the development of vaccines.
万方数据
维普
