The Preparation and Certification of Clostridium chauvoei Strain C54-1
In order to study the characteristics of C.chauvoei strain C54-1(CVCC60001),a batch of C.chauvoei strain C54-1 was prepared,and the strain morphology,biochemical characteristics,culture characteristics,serological characteristics,vacuum degree,purity,residual water,virulence and immunogenicity,16S rDNA of the strain were tested,and the growth promotion ability of the suitable medium for this strain was compared.The experimental results showed that the morphology,biochemical characteristics,culture characteristics,serological characteristics,vacuum degree,purity,residual water,virulence and immunogenicity of the lyophilized strain were in accordance with the quality standard of the Code of Veterinary Biological products regulations of the People's Republic of China(2000).16S rDNA was identified as C.chauvoei and the similarity was 99.93％.In the immunogenicity test,when lethal dose was used to attack the animals,the immune group could be protected by 4/4,which proved that the immune effect of the alum vaccine was good.The commercial clostridium culture medium was compared to confirm the possibility of commercial medium replacing anaerobic meat liver soup.This study provided a reference for the preparation and verification of C.chauvoei,and comparison studies using different media provided a research basis for the stable culture of the bacteria and the improvement of vaccine technology.
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