Preparation and Identification of the Monoclonal Antibodies against Mycoplasma bovis Strain NM001
This study was conducted to obtain the monoclonal antibodies against Mycoplasma bovis strain NM001 and to evaluate the characterization of the monoclonal antibodies.Six-week-old BALB/c mice were immunized with Mycoplasma bovis strain NM001,two hybridoma cell strains stably secreting antibodies against NM001,separately named as 2C5 and 7G3,were obtained by hybridoma technology and indirect ELISA.The antibody titers in cell supernatant of 2C5 and 7G3 were 6.4 × 103 and 1.2 × 104,respectively.Both monoclonal antibodies 2C5 and 7G3 were determined to be IgG1 category,and all light chains of 2C5 and 7G3 were belonged toλ chain.The monoclonal antibodies 2C5 and 7G3 were purified with antibody titers of 1.02 × 105 and 4.09 × 105,respectively.Moreover,results of indirect ELISA showed that both 2C5 and 7G3 can react with Mycoplasma bovis,but not react with the Mycoplasma agalactia,Mycoplasma capricolum subsp.capripneumoniae,Mycoplasma mycoides subsp.capri,Mycoplasma ovipneumoniae or Pasteurella multocida.The result of Western Blot showed that both monoclonal antibodies 2C5 and 7G3 can specifically recognize proteins of Mycoplasma bovis.These data suggest that monoclonal antibodies 2C5 and 7G3 can react specifically with Mycoplasma bovis,which would provide a basis for serological detection of Mycoplasma bovis.
万方数据
维普
