Determination of 62 Veterinary Drug Residues in Eggs by Solid-phase Extraction Combined with High-performance Liquid Chromatography Quadrupole Time of Flight Mass Spectrometry
Determination of 62 Veterinary Drug Residues in Eggs by Solid-phase Extraction Combined with High-performance Liquid Chromatography Quadrupole Time of Flight Mass Spectrometry
A method was established for the simultaneous determination of 62 veterinary drug residues in eggs using solid-phase extraction combined with high-performance liquid chromatography quadrupole time of flight mass spectrometry(HPLC-QTOF).The homogenizing eggs were extracted with a 90％formic acid acetonitrile aqueous solution,purified with PRiME HLB solid-phase extraction column,and dissolved again after nitrogen blowing and detected by HPLC-QTOF.62 veterinary drugs have good linear relationships(r2 ≥0.99)within the concentration range of 0.5～500 ng/mL with recoveries ranging from 71.2％to 113％,intraday variation coefficients ranging from 1.3 to 14.3％,intraday variation coefficients ranging from 4.2 to 18.3％,and the detection limit was 1.0～2.0 μg/kg,limit of quantitation was 2.0～5.0 μg/kg.This method is simple,fast,accurate,reliable and suitable for the simultaneous determination of 62 veterinary drugs in eggs.
关键词
鸡蛋/通过式固相萃取/高效液相色谱-四极杆飞行时间质谱/兽药残留
Key words
eggs/solid phase extraction purification/high performance liquid chromatography quadrupole time of flight mass spectrometry/veterinary drug residues
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