Determination of 62 Veterinary Drug Residues in Eggs by Solid-phase Extraction Combined with High-performance Liquid Chromatography Quadrupole Time of Flight Mass Spectrometry
A method was established for the simultaneous determination of 62 veterinary drug residues in eggs using solid-phase extraction combined with high-performance liquid chromatography quadrupole time of flight mass spectrometry(HPLC-QTOF).The homogenizing eggs were extracted with a 90％formic acid acetonitrile aqueous solution,purified with PRiME HLB solid-phase extraction column,and dissolved again after nitrogen blowing and detected by HPLC-QTOF.62 veterinary drugs have good linear relationships(r2 ≥0.99)within the concentration range of 0.5～500 ng/mL with recoveries ranging from 71.2％to 113％,intraday variation coefficients ranging from 1.3 to 14.3％,intraday variation coefficients ranging from 4.2 to 18.3％,and the detection limit was 1.0～2.0 μg/kg,limit of quantitation was 2.0～5.0 μg/kg.This method is simple,fast,accurate,reliable and suitable for the simultaneous determination of 62 veterinary drugs in eggs.
eggssolid phase extraction purificationhigh performance liquid chromatography quadrupole time of flight mass spectrometryveterinary drug residues
万方数据
维普
