In order to analyze and evaluate the uncertainty in the determination of florfenicol soluble powder,high performance liquid chromatography(HPLC)was used to determine the content of florfenicol soluble powder,C18 column was selected,Acetonitrile-Water-Glacial Acetic acid(100∶197∶3)was used as the mobile phase,and the detection wavelength was 224 nm.At the same time,a mathematical model for the determination of florfenicol soluble powder by HPLC was established.Analyze the standard uncertainty components introduced by various factors,evaluate the expanded uncertainty of the measurement results in the 95％confidence interval,and give the uncertainty evaluation result report.The results show that the uncertainty of content determination mainly comes from weighing,solution preparation and peak area determination.The standard uncertainty of content determination is 1.16％and the extended uncertainty is 2.32％.The content determination result is(97.3± 2.32)％,including factor k=2.This method is suitable for the uncertainty evaluation of the content determination of florfenicol soluble powder by HPLC,and can provide reference for the quality control and objective evaluation of florfenicol soluble powder.
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