A Comparative Study of the Determination of Zinc Baicalin Content by HPLC and Atomic Absorption Spectrometry
In order to compare the different methods for the determination of baicalin-zinc complex and establish a simpler and faster content determination method,HPLC method and atomic absorption spectrophotometry method were used to study the content determination of baicalin-zinc complex.The linear range of baicalin-zinc complex was 10.0~200.0 μg/mL(r=0.9999),and the precision(RSD=0.08%)was good by HPLC.The linear range of atomic absorption spectrometry was 0~2.0 μg/mL(r=0.9983),and the precision(RSD=0.13%)was good,but the pretreatment process was too complex and time-consuming.The HPLC method was simpler,faster and accurater,which could be used to determine the content of baicalin-zinc complex.