The purpose of this study was to construct a subunit vaccine of erysipelas with the dominant epitopes of SpaA of Erysipelothrix erysipelas in tandem,and to study its immunogenicity.The dominant epitopes of surface protection antigen A of Erysipelothrix erysipelas was expressed in tandem,and the recombinant protein was extracted and purified by affinity chromatography.The subunit vaccine was prepared and immunized mice and pigs which was challenged 21 days later.The erysipelas subunit vaccine with the dominant epitopes of SpaA in tandem was successfully prepared.2 μg recombinant protein can provide 100%protection for mice against 1000MLD virulent attack.100 μg recombinant protein can provide 100%protection against the attack of 1MLD virulent attack and 67%protection against the attack of 2MLD virulent attack for pigs.The erysipelas subunit vaccine with the dominant epitope of SpaA in tandem has good immunogenicity.
关键词
串联/SpaA/优势表位/猪丹毒/疫苗/免疫原性/攻毒保护
Key words
tandem/SpaA/dominant epitopes/Erysipelas/vaccine/immunogenicity/protection against chal-lenge
维普
万方数据