Determination of Tartaric Acid in Valnemulin Hydrogen Tartrate by Ion Chromatography
To establish an ion chromatography method with suppressed conductivity detection for the tartaric acid determination in the chemical reference standards the tartaric acid content of valnemulin hydrogen tartrate was determined by an Dionex IonPacTM AS19(4×250 mm)anion exchange column and Dionex IonPacTM AG18(4×50 mm)guard column,using suppressed conductivity detector.An isocratic eluent of 22 mmol/L KOH was chosen as mobile phase,flow rate was1.0 mL/min.Results manifested that a good linear relationship was observed within the range of 1.0～50.0 μg/mL(r =0.999,n =6).The limit of quantification was 0.5 μg/mL.The average spiked recovery was 98.07%at three concentration levels with RSD of 2.20%(n =9).Five batches of in valnemulin hydrogen tartrate samples from 3 manufactures were tested using this validated method,and the contents of tartaric acid were basically consistent with the theoretical values.The method is accurate and sensitive,which provides a reliable analytical method for the control of the proportion and content of the tartaric acid salts.
ion chromatographychemical reference standardstartaric acidvalnemulin hydrogen tartrate
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维普
