Quality of Horned gallnut and Belly gallnut from Different Producing Areas Evaluated by HPLC Characteristic Chromatogram Combined with Chemometrics
To use high-performance liquid chromatography(HPLC)to determine the HPLC characteristic fingerprints of two different forms of Galla chinensisfrom different producing areas,and to study the differences in effective components in different forms of Chinese gallnuts through cluster analysis and principal component analysis,lay the foundation for the quality control of gallnut.HPLC gradient elution method was used to determine the samples of horned gallnut and belly gallnut,and the chromatograms were recorded.The chromatograms were matched according to the ＂ Similarity Evaluation System for Traditional Chinese Medicine Chromatographic Fingerprints(2012 Edition)＂ to generate characteristic spectra of horned gallnut and belly gallnut,and similarity analysis was performed.Cluster analysis and principal component analysis(PCA)were conducted on the abdomen and horn polyploids using the chemometric software Origin 2018.The results showed that a common pattern of HPLC characteristic fingerprints for horned gallnut and belly gallnut was established,identifying four characteristic peaks:gallic acid,methyl gallate,1,2,3,6-tetra-O-glycerol,and 1,2,3,4,6-penta-O-glycerol.The similarity between Jiaobei and Beibei is low,and they can be divided into two categories through cluster analysis and principal component analysis.Compared with the HPLC fingerprint of the two,belly gallnut has 5 more peaks,with retention times of 20.3 minutes,26.0 minutes,27.6 minutes,54.6 minutes,and 75.9 minutes,and a retention peak of 49.1 minutes in relative position is missing.By analyzing the characteristic chemical components in Chinese gallnut and Tripterygium from different regions,it is shown that belly gallnut has more effective components than horned gallnut.By using similarity research,cluster analysis techniques,and principal component analysis,the two can be effectively distinguished,providing reference for the comprehensive evaluation and utilization of the quality of Chinese gallnut medicinal materials.
Chinese gallnutcharacteristic chromatogramprincipal component analysiscluster analysis
万方数据
维普
