Evaluating the uncertainty on determination of flunixin content in flunixin meglumine injection by liquid chromatography can be used to improve the accuracy of the test results and determine the key influencing factors in the test process.The flunixin content in flunixin meglumine injection was tested according to Chinese Veterinary Pharmacopoeia 2020 edition,and the factors affecting the uncertainty were analyzed.Referring to Evaluation of Measurement Uncertainty in Chemical Analysis JJF 1135-2005,Evaluation and Representation of Measurement Uncertainty JJF 1059.1-2012 and Guidance on Evaluating the Uncertainty in Chemical Analysis(CNAS-GL006:2019),a mathematical model for the uncertainty evaluation of flunixin content was built to evaluate the uncertain factors in the test.The sources of uncertainty were analyzed,and the combined relative standard uncertainty and expanded uncertainty were calculated.Uncertainty results of flunixin content in flunixin meglumine injection were expressed as(102.2±2.72)%,(k = 2,The confidence interval is 95%).The uncertainty of flunixin content in flunixin meglumine injection mainly comes from the preparation of the Instrument repeatability.
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