Preparation and Identification of Porcine Parvovirus Hemagglutination Inhibition Test Antigen,Positive Serum and Negative Serum
Porcine parvovirus(PPV)hemagglutination inhibition test antigen,positive serum and negative serum are indispensable for the efficacy test of PPV products,and it is very important for the quality control of PPV related biological products.The hemagglutination inhibition test antigen was prepared by simultaneous inoculation of PPV 7909 strain with PK-15 cells.The positive serum was prepared by emulsifying the antigens and immunizing guinea pigs,and the negative serum was prepared by non-immunized negative guinea pigs.Antigen,positive serum and negative serum are identified,the results show that the PPV hemagglutination inhibition test antigen HA titer was 1∶512;the positive serum HI titer was 1∶1024;and the negative serum HI titer<1∶8,all of the preparation have good specificity.Cross-reaction test was conducted by using the prepared hemagglutination inhibition test antigen,positive serum and the inactivated antigen,positive serum of different PPV vaccine strains,the results showed that the antigen and positive serum had good serological cross-reaction and could be used for unified evaluation of PPV products.
porcine parvovirushemagglutination inhibition test antigenpositive serumbiological productstest
万方数据
维普
