Determination of Andrographolide and Dehydroandrographolide in Siwei Chuanxinlian San by HPLC with Dual-wavelength
To establish a Dual-wavelength HPLC method for determination of andrographolide and dehydroandrographolide in Siwei Chuanxinlian San.Xbridge C18 column(4.6 mm×150 mm,5 μm)was used with methanol-water(52∶48)as mobile phase at a flow rate of 1.0 mL·min-1,and a column temperature 25℃.The determination wave length of andrographolide was 225 nm while dehydroandrographolide was 254 nm.The linear range was 12.7375 μg·mL-1 to 509.5 μg·mL-1(r2 =0.9994)and 3.4 to 136 μg·mL-1(r2 = 0.9995),and the average recovery was 98.8%and 99.9%with RSD of 1.0%and 0.8%,respectively.The method is rapid,accurate and sensitive,which provides a rapid and accurate technical means for the determination of andrographolide anddehydroandrographolide in Siwei Chuanxinlian San.
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