The Research of 199 Drugs and Metabolites Residues in Livestock And Poultry Muscle by UPLC-MS/MS
A UPLC-MS/MS method was established for the determination of 119 drugs and metabolites residues in livestock and poultry muscle.The samples were extracted with Mcllvaine-Na2 EDTA buffer and acetonitrile(2%formic acid),and the protein was deposited by high-speed centrifugation.Then the sample solutions were purified with EMR-Lipid extraction column.The mobile phase were water(0.1%formic acid)and acetonitrile(0.1%formic acid),and the samples were separated by C18 chromatography column.Ionization was performed using both positive and negative ion electrospray ionization(ESI+/ESI-)at the same time,applying the detection mode of multiple reaction monitoring(MRM).The method was quantified by matrix matched standard curves.Good linearities of the calibration curves were obtained with the range of matrix matched standard curves in blank pig,chicken,cattle and sheep muscle,the correlation coefficients(r)>0.990.The limits of detection of the method were 0.5～20 μg/kg.The recoveries were 60%～120%at the concentrations of LOQ～200 μg/kg.The intra-and inter-batch variation coefficients were both less than 20%.With good selectivity,accuracy and repeatability,the method can be used for simultaneous treatment and simultaneous detection of various drug residues.It will be of great significance to improve the efficiency of actual sample detection,better ensure the safety of animal food and protect human health.
UPLC-MS/MSlivestock and poultry muscleveterinary drug residues
万方数据
维普
