Determination of Gatifloxacin Residues in Beef by Liquid Chromatography-tandem Mass Spectrometry
To establish a method for determination of gatifloxacin in beef by liquid chromatography-tandem mass spectrometric(LC-MS/MS),beef samples were extracted with 80%(V/V)acetonitrile solution and then the extracts were purified by the Prime HLB solid phase extraction.The gradient elution was carried out with 0.1%(V/V)formic acid methanol and 0.1%(V/V)formic acid water.Positive ion scanning mode of electric spray ion source(ESI+),multiple reaction monitoring(MRM),internal standard method were applied for quantitative analysis.There was a good linear correlation between the peak areas and the concentrations of gatifloxacin at the range of 1-100 ng/mL(r2 =0.999),the limit of detection and limit of quantification was 1.0 and 2.0 μg/kg.The recovery rates were 64.2%-114.6%and the relative standard deviations were 5.5%-9.7%(n =6)at the addition levels of 2.0,4.0 and 50.0 μg/kg.This method is fast,accurate and sensitive,can be used for determination of gatifloxacin residues in beef.
liquid chromatography-tandem mass spectrometricbeefgatifloxacinresidues
万方数据
维普
