Detection of Imidocarb Residues in Cattle Tissues and Milk by High Performance Liquid Chromatography-Tandem Mass Spectrometry
A high performance liquid chromatography-tandem mass spectrometry method was established for the determination of imidocarb residues in cattle tissues(muscle,liver,kidney,fat)and milk.Imidocarb was enzymolized in sodium acetate buffer,and extracted with HCl solution.Then the sample solutions were purified with WCX SPE column.The mobile phase were formic acid solution(20 mM ammonium formate)and acetonitrile(0.3%formic acid),and the samples were separated by HILIC column.The residues was analyzed by positive ion electrospray ionization(ESI+)applying the detection mode of MRM and quantified by isotopic internal standard.Good linearities of the calibration curves were obtained with the range of 2.5～1000 ng/mL,the correlation coefficient R2>0.99.The limit of detection of imidocarb was10 μg/kg,and the limit of quantification of imidocarb was 20 μg/kg.The recoveries of imidocarb residues were 70.9%～109%,for cattle tissues(muscle,liver,kidney,fat)and milk at the concentrations of 20～4000 μg/kg.The intra-batch variation coefficients were 0.55%～9.59%,and inter-batch variation coefficients were 2.21%～12.1%.With high sensitivity,good accuracy and repeatability,the method can be applied for the determination of imidocarb residues in cattle tissues and milk.
cattle tissuesmilkimidocarbresidueshigh performance liquid chromatography-tandem mass spectrometry method
万方数据
维普
