Determination of Tilmicosin Residue in Edible Chicken Tissues by an Isotope-labeled Internal Standard Liquid Chromatography Tandem Mass Spectrometry
A liquid chromatography tandem mass spectrometry(LC-MS/MS)internal standard method for the detection of tilmicosin in four chicken edible tissues has been established.The samples were extracted with potassium dihydrogen phosphate buffer and acetonitrile,then cleaned up by C18 solid phase extraction columns,with 0.1%formic acid acetonitrile 0.1%formic acid water as the mobile phase,and was determined by liquid chromatography tandem mass spectrometry and quantified by internal standard method.The experimental results showed that there was a good linear relationship between the concentration range of 1 μg/kg to 100 μg/kg of tilmicosin in four chicken edible tissues,and the correlation coefficient r was greater than 0.99.The detection limit of tilmicosin in four chicken edible tissues is 0.5 μg/kg,and the quantitative limit is 1 μg/kg,when the concentration of timicosin in four tissues is 1～4800 μg/kg,the average batch-to-batch recovery rate is 94.6%～100.9%,the coefficient of variation within and between batches ranged from 1.11%to 5.06%.This method is suitable for the determination of tilmicosin content in four chicken edible tissues.
tilmicosinchicken edible tissuesLC-MS/MSinternal standard method
NETL
NSTL
万方数据
