Protective Mechanisms of Water Extract from Branches and Leaves of Clerodendrum inerme on Fever Rabbits induced by Lipopolysaccharide
Aim to study the antipyretic activity of water extract from branches and leaves of Clerodendrum inerme and its mechanisms.Rabbit fever model induced by auricular vein injection of Lipopolysaccharide(LPS,4 μg/kg),and 1 h later,water extract(1,2 and 4 g/kg)were orally fed with 5 mL/kg once,their rectal temperature checked,their liver function and endogenous pyrogen indexes in serum analysed,their liver and hypothalamus histopathologic analysed by HE staining,and their expressoin of liver COX-2 and hypothalamic TLR4,NF-κB,COX-2 analysed by immunohistochemical staining.The results showed that all water extract administration groups could significantly decrease rectal temperature(P<0.05 or P<0.01),serum AST,ALT,TNF-α,IL-6,IL-1β,PGE2 level(P<0.05 or P<0.01),their hepatic and hypothalamic histological aberrations obviously alleviated,their expression levels of liver COX-2 and hypothalamic TLR4,NF-κB,COX-2 significantly decreased(P<0.05 or P<0.01).Water extract from branches and leaves of Clerodendrum inerme has good antipyretic activity,it may related to improving the hepatic function,decreasing blood endogenous pyrogen,and downregulating the expression of liver COX-2 and hypothalamic TLR4,NF-κB,COX-2.
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