摘要
研究苦郎树枝叶水提物的解热作用及其机制.采用耳缘静脉注射脂多糖(LPS,4 μg/kg)复制家兔发热模型,水提物剂量设定为 1、2、和 4 g/kg,按 5 mL/kg单次灌胃给药,药后检测肛温,测定血清肝功能和内生致热原指标水平,HE染色法检测肝组织和下丘脑组织病理学改变,免疫组化染色法检测肝组织COX-2 和下丘脑TLR4、NF-κB、COX-2 表达水平.结果表明,水提物各剂量组均可显著降低肛温(P<0.05 或P<0.01),降低血清ALT、AST、TNF-α、IL-1β、IL-6 和PGE2水平(P<0.05 或P<0.01),明显减轻肝组织和下丘脑组织病理学损伤,降低肝组织COX-2和下丘脑TLR4、NF-κB、COX-2 表达水平(P<0.05 或P<0.01).苦郎树枝叶水提物具有良好的解热作用,其机制可能与增强肝功能、消除血液内生致热原及下调肝组织COX-2 和下丘脑TLR4、NF-κB、COX-2 表达水平有关.
Abstract
Aim to study the antipyretic activity of water extract from branches and leaves of Clerodendrum inerme and its mechanisms.Rabbit fever model induced by auricular vein injection of Lipopolysaccharide(LPS,4 μg/kg),and 1 h later,water extract(1,2 and 4 g/kg)were orally fed with 5 mL/kg once,their rectal temperature checked,their liver function and endogenous pyrogen indexes in serum analysed,their liver and hypothalamus histopathologic analysed by HE staining,and their expressoin of liver COX-2 and hypothalamic TLR4,NF-κB,COX-2 analysed by immunohistochemical staining.The results showed that all water extract administration groups could significantly decrease rectal temperature(P<0.05 or P<0.01),serum AST,ALT,TNF-α,IL-6,IL-1β,PGE2 level(P<0.05 or P<0.01),their hepatic and hypothalamic histological aberrations obviously alleviated,their expression levels of liver COX-2 and hypothalamic TLR4,NF-κB,COX-2 significantly decreased(P<0.05 or P<0.01).Water extract from branches and leaves of Clerodendrum inerme has good antipyretic activity,it may related to improving the hepatic function,decreasing blood endogenous pyrogen,and downregulating the expression of liver COX-2 and hypothalamic TLR4,NF-κB,COX-2.
基金项目
广西壮瑶药重点实验室项目(桂科基字[2014]32号)
壮瑶药协同创新中心项目(桂教科研[2013]20号)
广西壮族自治区民族药资源与应用工程研究中心项目(桂发改高技函[2020]2605号)
广西中医药重点学科壮药学项目(GZXK-Z-20-64)
广西壮族自治区重点研发计划(桂科AB21196016)
广西科技基地和人才专项(桂科AD21238031)
国家科技期刊平台
NSTL
万方数据