Establishment of a Quality Control Method based on Network Pharmacology and HPLC Method for the Quality Control of Multi-indicator Components in Compound Shi-lin-tong Tablets for pets
To establish a multi-indicator method for the determination of the content of Compound Shi-lin-tong Tablets for pets.Screening the key active ingredients of Compound Shi-lin-tong Tablet for the treatment of urolithiasis and cystitis by using network pharmacological methods,combining with the relevant literature and the relevant requirements of Chinese Pharmacopoeia,to determine the indicator ingredients.The method was established for the determination of the content of the indicator components by HPLC analysis using a CAPCELL PAK C18 MG(S-5)4.6×250 mm column at 30℃ with an injection volume of 10 μL,a flow rate of 1.0 mL/min,and a detection wavelength of 335 nm,with a gradient elution using acetonitrile-0.1%formic acid in water as the mobile phase.Ten key components such as quercetin,caffeic acid,and chlorogenic acid were screened by network pharmacology.Six index components,neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,caffeic acid,charophylloside and isochlorophylloside,were selected in conjunction with the Chinese Pharmacopoeia and related literature.The methodological results of the content determination showed:The linearity of the components was good(r≥0.999);Good precision,RSD of 0.03%～0.21%;Repeatability was good with RSD of 0.70%to 2.02%;The stability of the test material was good over 24 h with RSD of 0.30%～2.02%;The average spiked recoveries(RSD)ranged from 98.31%to 104.31%(0.36%to 2.28%);The levels of the six components in the three batches of samples ranged from 0.990 to 1.434 mg·g-1,1.533 to 2.083 mg·g-1,1.311 to 2.023 mg·g-1,0.332 to 0.353 mg·g-1,1.594 to 1.768 mg·g-1,and 0.787 to 1.057 mg·g-1,respectively.The content determination method has good reproducibility and stability,and can be used for the quality analysis of Compound Shi-lin-tong Tablets for pets.
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