Simultaneous Detection of 48 Doping Agents in Animal Urine by High Performance Liquid Chromatography-tandem Mass Spectrometry
A high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method was established for the simultaneous detection of 48 stimulants such as β2-agonists,β-blockers,diuretics,glucocorticoids,and anabolic agents in animal urine.The sample was extracted with 1%formic acid acetonitrile,added sodium chloride to make the layers,dried under nitrogen at 40℃,redissolved in acetonitrile aqueous solution(V/V,5∶5,containing 0.1%formic acid),and separated by C18 chromatographic column.48 kinds of stimulants such as clenbuterol can achieve good separation and good repeatability.When the spiked level is the limit of quantification,the recovery rate of the target compound is between 60%and 120%,and the relative standard deviation is 1.0%to 19.3%.The method is simple,fast and accurate,which greatly improves the detection efficiency.It has been applied to routine doping detection in laboratories,and provides strong technical support for ensuring the smooth progress of sports events.
high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)animal urinedoping
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