Determination of Total Polysaccharides,Total Flavonoids and Four Action Components in Aphrodisiac Powder
To establish a method for the determination of total polysaccharides and total flavonoids in aphrodisiac powder,and to establish a method for the simultaneous determination of the action components of icaritin A,icaritin B,icaritin C and icariin,so as to provide a comprehensive basis for the quality control of aphrodisiac powder.The content of total polysaccharides and total flavonoids in aphrodisiac powder was determined by colorimetry,and the main components in aphrodisiac powder were analyzed and their contents were determined by high performance liquid chromatography(HPLC).There were some differences in the contents of the main chemical components in aphrodisiac powder from different manufacturers.The average content of total polysaccharides in aphrodisiac powder was 3.35%,the average content of total flavonoids was 1.30%,and the average contents in aphrodisiac powder were 0.0137%,0.0131%,0.1106%and 0.0764%for Icaritin A,Icaritin B,icaritin C and icariin,respectively.A method for the determination of total polysaccharides,total flavonoids and four action components in aphrodisiac powder was established,which has good stability and reproducibility and can be used as a standard for quality control of aphrodisiac powder.
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