Effects of Maduramicin Ammonium on Transcriptome and Ultrastructure of Myoblasts
This study was set to investigate the effect of maduramicin ammonium on gene expression and ultrastructure in myoblasts.C2C12 cells were treated with maduramicin ammonium,the cell growth was recorded in the cell real-time analyzer;transcriptome sequencing was performed using the Illumina HiSeq platform to screen differentially expressed genes and perform gene ontology enrichment analysis;the vacuoles were observed and photographed under a microscope;the ultrastructure were observed and photographed under a transmission electron microscope.The results showed that maduramicin ammonium inhibited the growth of C2C12 cells in a concentration-and time-dependent manner.After treatment with 1 μM maduramicin ammonium for 8 h,there were 2450 differentially expressed genes,including 1411 down-regulated genes and 1039 up-regulated genes.gene ontology analysis showed that the differentially expressed genes were significantly enriched in vacuole,DNA repair,positive regulation of transferase activity,etc.Intracellular vacuoles increased significantly after treatment with 1 μM maduramicin ammonium for 8 h.After treatment with 1 μM maduramicin ammonium for 24 h,the organelles were obviously swollen,the mitochondrial crista structure was medullated,and ribosome degranulation was attached to the surface of the rough endoplasmic reticulum.The results suggested that maduramicin ammonium induced vacuolation of C2C12 cells and destroyed the structures of organelle,resulting in cell growth inhibition and its toxicity to myoblasts.This study reveals that intervening cell vacuolization is an important means to prevent and treat maduramicin ammonium poisoning.
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