Research Progress on Laboratory Detection Methods for ORF
Orf is an epitheliophilic zoonotic infectious disease caused by the sheep infectious pustular dermatitis virus(ORFV).This disease is prevalent both domestically and internationally,causing huge economic losses to the sheep farming industry.Therefore,fast and efficient detection methods play a crucial role in the prevention and treatment of orf.The commonly used laboratory detection methods for orf include virus isolation and identification,virus neutralization assay(VNT),enzyme-linked immunosorbent assay(ELISA),immunofluorescence assay(IFA),agar diffusion assay(ATD),Western blotting(WB),side flow immunoassay(LFIA),polymerase chain reaction(PCR),real-time fluorescence quantitative PCR(qPCR),etc.This article provides an overview of the research progress on laboratory detection methods for ORFV,providing a basis for rapid and accurate diagnosis of ORFV.
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