Construction of Virus-Like Particles of H9N2 Avian Influenza Virus
In order to construct virus-like particles of H9N2 avian influenza virus,this study used the proteins of hemagglutinin(HA),neuraminidase(NA)and matrix protein(M1)to construct two recombinant baculoviruses rBV-HA-M1 and rBV-NA,through the Bac-to-Bac baculovirus expression system.The immune activity of the recombinant proteins were identified by IFA and Western-blot,then the Sf9 insect cells were co-infected with rBV-HA-M1 and rBV-NA at the optimal infection complex ratio.The co-infected Sf9 cells were concentrated to obtain the virus-like particles.The virus-like particles were measured for hemagglutination titers and transmission electron microscopy.The results of transmission electron microscopy showed that the expressed HA-M1 and NA recombinant proteins could self-assemble to form virus-like particles in Sf9 cells.The virus-like particles were able to agglutinate 1% of chicken red blood cells,and the titer was 1:64.The results of this study lay the foundation for further research on the virus-like particle vaccine of H9N2 virus.
avian influenza virussubtype H9N2virus-like particlesBac-to-Bac baculovirus expression system
NETL
NSTL
万方数据
