板蓝根注射液特征图谱及其质量相关性研究
Study on the Characteristic Graph and Quality Correlation of Banlangen Injection
周景瑞 1冉江 1姜玲玲 1雷露 1许浩翔 1艾蓉 1罗文菊 1张琴 1章厉劼 2余波1
作者信息
- 1. 贵州省农业科学院 畜牧兽医研究所,贵阳,550000
- 2. 贵州省兽药饲料检测所,贵阳,550000
- 折叠
摘要
建立板蓝根注射液尿苷、鸟苷、(R,S)-告依春、腺苷含量检测方法及指纹图谱研究.采用高效液相色谱法,色谱柱:Eclipse Plus C18 柱(4.6 mm×250 mm,5μm),检测波长250 nm,以甲醇(A)-水(B)为流动相,梯度洗脱(0～3 min,95%B;3～20 min,95%B～90%B;20～40 min,90%B～30%B;40～42 min,30%B～95%B;42～50 min,95%B),流速为 1.0 mL/min,柱温箱为30℃,进样量10 μL.该实验操作简单、快捷,结果准确,适用板蓝根注射液尿苷、鸟苷、(R,S)-告依春、腺苷含量的测定,建立指纹图谱,对板蓝根注射液进行质量评估.
Abstract
Objective:To establish a method for detecting the content of uridine,guanosine,(R,S)-goitrin,and adenosine in Banlangen injection and to study their fingerprints.High performance liquid chromatography was used,with Eclipse Plus C18 column(4.6 mm×250 mm,5 μm)Detection wavelength:250 nm,using methanol(A)-water(B)as the mobile phase,gradient elution(0～3 min,95% B;3～20 min,95% B～90% B;20～40 min,90% B-30% B;40-42 min,30% B～95% B;42～50 min,95% B),flow rate:1.0 mL/min,column temperature chamber:30℃,injection volume:10 μL.The experimental operation is simple,fast,and the results are accurate.It is suitable for the determination of uridine,guanosine,(R,S)-goitrin e,and adenosine content in Banlangen injection,and the establishment of a fingerprint for the quality evaluation of Banlangen injection.
关键词
板蓝根注射液/尿苷/鸟苷/(R,S)-告依春/腺苷/指纹图谱Key words
Banlangen injection/Uridine/Guanosine/(R,S)-goitrin/Adenosine/fingerprint引用本文复制引用
基金项目
贵州省科技厅项目(黔科合成果[2021]一般039)
&&(黔科合成果[2022]一般008)
贵州省农业农村厅项目(GZCYTX-0302)
出版年
2024
国家科技期刊平台
NSTL
万方数据