Effect of Different Processes of Banqing Granules on Anti-inflammatory Performance of White Feather Broilers under Lipopolysaccharide Stress
The objective of this study was effect of different processes of Banqing granules on anti-inflammatory performance of white feather broilers under Lipopolysaccharide stress.The thin layer identification of amino acids,organic acids,indirubin and(R,S)-geuichun in the extracts of different solvents was determined.The contents of(R,S)-geuichun,adenosine and uridine were determined to determine the quality of the products.The effects of the extracts of different solvents of geuiqing on endotoxin degradation in vitro were determined by quantitative chromogenic matrix method.At the same time,the effects of 30% ethanol extract on endotoxin degradation and ant-inflammation in white feather broilers were determined.The results showed that the amino acid and indirubin control samples extracted with 30% ethanol had large spots and dark colors.The content of uridine in the indigo extracted with 30% ethanol solvent was the highest,the content of water extracted indigo(R,S)-geuichun and adenosine was the highest.The indigo extracted with 30% ethanol had the best endotoxin degradation effect in vitro,and had a significant internal toxicity degradation effect in vivo.At the same time,It also inhibited the increase of serum IL-1β,IL-6 and TNF-α cytokines induced by lipopolysaccharide.The results indicated that the 30% ethanol extract of Banqing had significant endotoxin degrading and anti-inflammatory effects in vitro and in vivo.
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