高效液相色谱法测定白术中白术内酯Ⅰ、Ⅱ、Ⅲ的含量
Determination of Atractylenolide Ⅰ,Ⅱ and Ⅲ in Rhizoma Atractylodis Macrocephalae by High Performance Liquid Chromatography
黄思勇1
作者信息
- 1. 恩施职业技术学院,湖北恩施 445000
- 折叠
摘要
本实验建立了高效液相色谱技术(HPLC)测定白术中白术内酯Ⅰ、Ⅱ、Ⅲ的含量.采用Hypersil GOLD(250 mm×4.6 mm,5um),以乙腈为流动相A、以0.1%磷酸溶液为流动相B进行梯度洗脱,流速1.0 mL/min,白术内酯Ⅰ检测波长为 276 nm,白术内酯Ⅱ、白术内酯Ⅲ检测波长为220 nm,柱温为35℃,进样体积为20 μL.白术内酯Ⅰ在0.10～1.60 μg范围内线性关系良好,平均回收率为99.29%(RSD=2.25%);白术内酯Ⅱ在0.10～1.60 μg范围内线性关系良好,平均回收率为100.07%(RSD=2.59%);白术内酯Ⅲ在0.10～1.60 μg范围内线性关系良好,平均回收率为100.43%(RSD=2.20%).结果表明该方法分离度好、准确度高、重现性好,可测定白术中白术内酯Ⅰ、Ⅱ、Ⅲ的含量.
Abstract
This experiment established a high-performance liquid chromatography(HPLC)technique to determine the content of atractylenolide Ⅰ,Ⅱ and Ⅲ in Rhizoma Atractylodis Macrocephalae.Adopting Hypersil GOLD(250 mm×4.6 mm,5um),using acetonitrile as mobile phase A and 0.1％phosphoric acid solution as mobile phase B for gradient elution,with a flow rate of 1.0 mL/min.The detection wavelength of atractylenolide I was 276 nm,atractylenolide Ⅱ and Ⅲ were 220 nm,and the column temperature was 35℃.The injection volume was 20 μL.The linear relationship of atractylenolide I within the range of 0.10 to 1.60 μg is good,with an average recovery rate of 99.29％(RSD=2.25％);The linear relationship of atractylenolide Ⅱ within the range of 0.10 to 1.60 μg is good,with an average recovery rate of 100.07％(RSD=2.59％);The linear relationship of atractylenolide Ⅲ within the range of 0.10 to 1.60 μg is good,with an average recovery rate of 100.43％(RSD=2.20％).The results indicate that this method has good separation,high accuracy,and good reproducibility,and can be used to determine the content of atractylenolide Ⅰ,Ⅱ and Ⅲ in Rhizoma Atractylodis Macrocephalae.
关键词
白术/白术内酯Ⅰ/白术内酯Ⅱ/白术内酯Ⅲ/含量Key words
Rhizoma Atractylodis Macrocephalae/atractylenolide Ⅰ/atractylenolide Ⅱ/atractylenolide Ⅲ/content引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据