Uncertainty Evaluation for the Determination of Tilmicosin Residue in Swine Muscle by High Performance Liquid Chromatography Spectrometry
According to"Announcement No.1025-10-2008 of the Ministry of Agriculture of determination of tilmicosin residue in swine muscle by high performance liquid chromatography spectrometry",the uncertainties of tilmicosin residues in swine muscle were evaluated.Five evaluation components,including standard solution,sample weighing,sample solution,sample solution peak area and recovery were studied.Results showed that the relative combined standard uncertainties of determination of tilmicosin were 3.09 ×10-2,5.78 × 103,4.00 × 10-3,2.31× 10-2 and 1.34 × 10-2,respectively.Three evaluation components of standard solution,sample solution peak area and recovery had greater contribution in the combined uncertainty of tilmicosin residue.In this study,the detection results of tilmicosin in swine muscle samples in 95％confidence interval could be expressed as tilmicosin 71.6±5.92 μg/kg(k=2).The uncertainty assessment result provided the technical supplement for the accuracy and reliability of the determination of tilmicosin residue in swine muscle by high performance liquid chromatography spectrometry.
high performance liquid chromatography spectrometryswine muscletilmicosin,residueuncertainty
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