Study on Quantitative Detection of PCV2 Cap Antigen by Modified RIHA Method
To improve the stability and accuracy of the quantitative detection of the PCV2 Cap antigen using the reverse indirect hemagglutination assay(RIHA),the RIHA method has been modified as follows:1)Dilute the test sample into several appropriate dilutions for RIHA;2)Record agglutination in the test wells at 100％,75％,50％,25％and 0％as 4,3,2,1 and 0 points respectively,and sum the scores of all test wells for each dilution of the test sample;3)Perform the same method to test the reference standard and sum its scores;4)Compare the total scores of the test sample and the reference standard to obtain the antigen content of the test sample.The modified RIHA method was used for stability study and compared with the ELISA method.The results showed that the intra-batch difference of the modified RIHA method was 6.8％～19.4％,the inter-batch difference was 11.4％～23.6％,and the coefficient of variation for testing the same sample was 8.5％,and all of them were superior to the conventional RIHA method.The modified RIHA method was used to test 30 PCV2 Cap antigen samples before and after purification,and the results were compared with those obtained from the ELISA method,yielding a correlation coefficient of 0.9567.This indicates that the modified RIHA method for quantitative detection of PCV2 Cap antigen is stable and correlates well with the ELISA method.It can be used for quantitative detection in the production of PCV2 Cap antigens and also for rapid quantitative detection of PCV2 Cap antigen content in virus seed screening and production process optimization.
PCV2 Cap antigenmodifiedreverse indirect hemagglutination assay(RIHA)ELISAquantita-tive detection
万方数据
维普
