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HPLC测定兽用穿琥宁注射液中的有关物质

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建立一种HPLC法测定兽用穿琥宁注射液中的有关物质.采用Inertsil ODS-35μm(4.6 mm×250 mm)色谱柱;以0.1%磷酸二氢钾溶液(用磷酸调节pH值至2.5±0.1)为流动相A,以乙腈为流动相B,梯度洗脱;检测波长为251 nm.该方法专属性、稳定性、耐用性、重现性等良好,灵敏度高.该方法适用于兽用穿琥宁注射液中有关物质的测定.
Determination of the Related Substances of Potassinm Veterinary Dehydro and Rographolide Succinate Injection by HPLC
To establish an HPLC method to determine the relevant substances in veterinary water injection.Use Inertsil ODS-35 μm(4.6 mm × 250 mm) column;use 0.1% potassium dihydrogen phosphate solution (adjust pH with phospic acid to 2.5±0.1) as mobile phase A,acetonitrile as mobile phase B;gradient elution;detection wavelength is 251 nm.Results:The method has good specificity,stability,durability,reproducibility and high sensitivity.This method is suitable for the determination of related substances in animal water injection.

veterinary application injectionrelated substanceshigh performance liquid chromatography

章文丽、李鑫、马春花、余佳欣、杨涵月、李诗、董可

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四川育强本草生物技术有限公司,成都611100

兽用穿琥宁注射液 有关物质 高效液相色谱法

2024

中国兽药杂志
中国兽医药品监察所

中国兽药杂志

CSTPCD
影响因子:0.49
ISSN:1002-1280
年,卷(期):2024.58(12)