Determination of the Related Substances of Potassinm Veterinary Dehydro and Rographolide Succinate Injection by HPLC
To establish an HPLC method to determine the relevant substances in veterinary water injection.Use Inertsil ODS-35 μm(4.6 mm × 250 mm) column;use 0.1% potassium dihydrogen phosphate solution (adjust pH with phospic acid to 2.5±0.1) as mobile phase A,acetonitrile as mobile phase B;gradient elution;detection wavelength is 251 nm.Results:The method has good specificity,stability,durability,reproducibility and high sensitivity.This method is suitable for the determination of related substances in animal water injection.