Study on the Efficacy of Shugansan on a Rat Model of Chronic Hepatitis
Investigate the therapeutic effect of Shugan Powder,a traditional Chinese medicine compound,on chronic hepatitis in rats.A chronic hepatitis rat model was established using subcutaneous injection of 40％carbon tetrachloride (CCl4) solution.Sixty male Sprague-Dawley rats were randomly divided into control,model,low,medium,high dose Shugan Powder groups,and positive control groups,with 10 rats in each group.Except for the control group,the other groups were injected subcutaneously with 1.0 mL/kg of 40％ CCl4 solution twice a week for 10 weeks;simultaneously,from the first day of the experiment,they were given medication by gavage.Each treatment group was given the corresponding dose of the drug solution at 10 mL/kg by gavage,while the control and model groups were given an equal volume of pure water by gavage,once a day for 10 weeks.During the trial,the rats' appetite,condition,and mental state were observed.After the last administration,the rats were fasted for 12 hours before blood was drawn and dissection was performed.The expression of alanine aminotransferase (ALT) and aspartate aminotransferase (AST),type Ⅲ collagen (Col-Ⅲ) and type Ⅳ collagen (Col-Ⅳ),interleukin-1β(IL-1β),and interleukin-6 (IL-6) in the serum of rats from each group was detected,the liver organ coefficient was calculated,and the liver tissue pathology was observed.The results showed that compared with the control group,the model group had significantly or markedly increased levels of ALT,AST,Col-Ⅲ,Col-Ⅳ,IL-1β,IL-6,and liver organ coefficient in the serum (P<0.05 or P<0.01),and obvious vacuolar degeneration in the liver tissue;compared with the model group,all dose groups of Shugan Powder and the positive control group significantly or markedly reduced the levels of serum ALT,AST,Col-Ⅲ,Col-Ⅳ,IL-1β,IL-6,liver organ coefficient,and alleviated the degree of liver tissue lesions.In summary,the traditional Chinese medicine compound Shugan Powder has a significant therapeutic effect on the chronic hepatitis rat model.
万方数据
维普
