In order to ensure the nutrition and health of bee-pollen and improve the efficiency of bumblebee rearing,effects of disinfection for bee-pollen on Bombus breviceps were evaluated.Bee-pollen from the same source were treated as follows:control group(Ⅰ no disinfection)and disinfection groups(Ⅱ irradiated by 60Co,Ⅲ filled with ozone,Ⅳ steam heating at atmospheric pressure and Ⅴ heated at 60℃ under ultraviolet irradiation).The difference of colonies reproductive characteristics was analyzed by the data about development and health status of colonies after feeding.The results showed that every colony could lay eggs.Bee-pollen consumption from group Ⅳ(6.58±1.08 g)was significantly higher than control group and group Ⅴ(P<0.05).However,the number of larval ejections were markedly higher than the other four groups(P<0.05)and adult male was not produced.Compared with control group,bee-pollen consumption,development period and number of offspring in group Ⅱ,Ⅲ and Ⅴ were no significance(P>0.05).But at the duration of first drone emergence,group Ⅲ was shorter than control group(P<0.05).In terms of colony quality,the colonies fed with sterile bee-pollen were not infected with fungi,while the infection rate in the control group was 16.67%.The birth weight of drones in groups Ⅱ and Ⅴ was significantly higher than that in control group.In conclusion,the disinfection(irradiated by 60Co,filled with ozone and heated at low-temperature under ultraviolet irradiation)of bee-pollen could ensure the safety and nutrition of bee-pollen.Using it to rear micro-colonies will reduce the risk of mycosis infection and improve the efficiency of breeding.This study will be helpful for further studies on feed optimization and mass rearing of B.breviceps.
Bombus brevicepsfeedirradiationheatingozonecolony development