Apis cerana cerana foragers may contact Beauveria bassiana which has been widely used to control agricultural and forestry pests.In order to explore the effects on A.c.cerana foragers,the conidia suspensions at different concentrations,105,106,and 107spores/mL were spread on bottom filter paper of the cage or fed to foragers respectively at lab,kept under 30℃,60％RH.The results showed that after 14 days of exposure to B.bassiana(105,106,107spores/mL),the adjusted mortality rates of the three groups were 36.4％,68.2％,100％,respectively,and the survival curves were significantly lower than those of the control group(p＜0.0001),indicating a dose-toxic effect relationship.These results indicate that B.bassiana has strong toxicity to foragers.The survival curve of worker bees fed with 105,106,107spores/mL sugar solution did not correspond to the dose,indicating that there was no obvious toxicity after feeding the B.bassiana spores.It is suggested that during the application of B.bassiana,it is advisable to avoid bee apiaries,or flowering nectar sources,or colonies transfer to other safe sites,which may reduce the contact toxicity to A.c.cerana.
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