Varroa mites are one of the most significant pests affecting Western honeybees.In recent years,despite various methods being developed to control mites(such as smoke fumigation,brushing to kill mites,breeding mite-resistant bee species,and ultrasound mite control),the effectiveness of mites control remains less than ideal.This study explores the large-scale production of drone pupae during the main honey flow season,using an invented pupae harvesting machine to collect drone pupae and simultaneously trap Varroa mites.The results demonstrate that this method not only significantly reduces the parasitic levels of Varroa mites,providing a buffer time for chemical mite control,but also decreases residues in bee products,offering valuable insights for beekeepers in Varroa mites management.