Study on Rapid Determination Technique of Cistanche deserticola Seed Viability
Traditional single-factor experiment, multi-factors interacted experiment, and orthogonal experiment were applied to improve the pretreatment methods, concentration and pH value of 2,3,5-triphenyl tetrazolium chloride (TTC) solution in the seed viability determination process. A rapid determination technique of Cistanche deserticola seed viability was primarily established by TTC test, which progress was simplified and reaction time was cut short. The optimum pH value and TTC solution concentration was 6.4 and 0. 3% -1.0% respectively at 38 ℃.