Study on Endogenesis Inhibitory Substances in Seed of Astragalus membranaceus
Objective:To investigate the activity and the location of the endogenesis inhibitory substances in seed of Astragalus membranaceus.Method:For obtaining extracts of different solvent,concentrations and extracting times,extraction method was adopted in different tissues of Astragalus membranaceus seeds.Biological activities were determined by wheat and Brassica sp seed.Result:The extract dramatically inhibited the germination and the growth of wheat and Brassica sp seed and the inhibitory activity was positively related to the concentration of the extracts.When the concentration reached 0.20 g/mL,wheat and Brassica sp seed did not germinate.Distinct difference in inhibitory activity in extracts of different solvent from Astragalus membranaceus seeds were found,of which the water-soluble extracts' inhibitory activity was more significant than alcohol-soluble extracts.With the increased extracting times,the inhibitory activity showed a trend of weakening.The seed coat and kernel both had inhibitory substances,and the latter was stronger than the former.Conclusion:Highly active inhibitory substances were found in the seed of Astragalus membranaceus,and most of them were water-soluble ingredients.The active inhibitory substances in seed kernel was higher than seed coat in Astragalus membranaceus seeds.
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