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人工老化和修复处理对小麦种子活力及生理特性的影响

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种子在贮藏过程中,会出现老化现象,使种子活力下降,生理特性发生改变.本实验采用高温高湿(T=52.3℃,RH=80%)人工老化方法处理小麦种子,并以500 mg/L抗坏血酸(AsA)、20%聚乙二醇(PEG) 6000、500 mg/L赤霉素(GA3)3种溶液进行老化种子的修复,人工老化及修复后的种子经室内发芽试验,分别测定种子发芽指标、种子活力、过氧化物酶(POD)活性、丙二醛(MDA)含量变化情况.结果表明,经人工老化处理后,小麦种子的发芽势下降2.27%,发芽率下降2.67%,种子活力(TTC还原强度)下降7.29%,这三者虽都有所下降,但未达到显著水平(p<0.05);而老化种子的活力指数和发芽指数下降显著,分别较未老化处理种子下降35.86%和51.51%.PEG修复处理能显著提高老化种子的发芽指数;GA3修复处理对老化种子活力指数的修复效果最好;AsA和GA3修复处理使老化种子的MDA含量显著降低,但未达到未经处理种子的MDA含量水平;PEG、GA3和AsA修复处理对POD酶活性都有一定程度的提高,但影响不显著.
Effects of Artificial Accelerated Aging and Repairing Process on Seed Vigor and Physiological Characteristics of Wheat Seeds
With the extension of storage time,the seeds aging occur,the activity decreases,and physiological characteristics change.In this study,high temperature and high humidity (T =52.3 ℃,RH =80%) artificial aging method was used to deal with the wheat seed,and 500 mg/L ascorbic acid (AsA),20 % polyethylene glycol (PEG) 6000,500 mg/L gibberellinic hormone (GA3) three solutions were used to repair aging seeds.Seeds artificial aging and repaired by the indoor germination test,seed germination index,seed vigor,peroxidase (POD) activity,malondialdehyde (MDA) content were measured.The results showed that wheat seed germination potential declined 2.27%,germination rate decreased by 2.67%,seed vigor (TTC reducing intensity) fell 7.29% by artificial aging,all three had declined,but did not reach a significant level (p<0.05);and compared with the unaged seeds,the aging of seed vigor index and germination index decreased 35.86% and 51.51%.PEG repair treatment could significantly improve the germination index;The repair effect of GA3 repair on aging seed vigor index was the best.;AsA and GA3 repair treatment significantly reduced the content of MDA in aged seeds,but did not reach the MDA content of untreated seeds.PEG,GA3 and AsA repairing had improvement for POD enzyme activity to some extent,but the effect was not significant.

wheatagingrepairseed vigorphysiological characteristics

丁忆然、李卫华、柴亚茹、冯宽、赵云、张辉、洪雪梅

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石河子大学农学院,新疆石河子832000

石河子农业科学院作物所,新疆石河子832000

小麦 老化 修复 种子活力 生理特性

新疆兵团科技局重点科技项目大学生研究训练计划项目

2011 BA 002SRP 2014190

2016

种子
贵州省种子管理站 贵州省种子学会 中国种子协会

种子

CSTPCD北大核心
影响因子:0.502
ISSN:1001-4705
年,卷(期):2016.35(4)
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