The Effect of IAA in Lettuce Seed on Cold Resistance Under Low Temperature Stress During Programmed Cooling
Seed is an important survival strategy for hibernating,and seed cryopreservation is the main way for germ plasm resources conservation.In this paper,choosing lettuce seed as the object,and using programmed cooling apparatus to create different cooling rates (3 ℃/h and 60 ℃/h),study the mechanism of IAA responding to low temperature stress.The results showed that compared with fast cooling(60 ℃/h),slow cooling (3 ℃/h) is beneficial to the increasing of IAA;using IAA can reduce frozen hurt of the lettuce seed during the program cooling process,especially in 200μm IAA in slow cooling;regardless of cooling rate,SOD activity and proline content will increased significantly,and SOD activity was directly proportional to the IAA concentration,while proline content was having exactly the opposite effect;different cooling rates,and the concentrations of IAA can also influence to cold relative genes:ICE 1,HOS 1.
IAAlettuce seedcold hardinesslow temperature stress