Cloning and Sequential Analysis of Transcription Regulator Factor WRKY71 from Arabidopsis
The RT-PCR method was used in this study,Cloning of the transcription factor WRKY71 from the leaf of Arabidopsis thaliana from the model plant,Sequencing and blast homology analysis,The results show that the sequence is basically consistent with the AtWRKY71 sequence of GenBank landing,The 57 bp position is changed from G to C only in the downstream of the starting codons,But it does not change the encoding of amino acids,It shows that the transcriptional regulator AtWRKY71 has been cloned successfully,and lay the foundation for the follow-up function identification.