菟丝子和大菟丝子形态组织研究与分类学意义
Morphological Organization and Taxonomic Significance of Cuscutae Semen and Cuscutae Japonicae Semen
余波 1瞿添添 1刘应蛟 1吴志瑰 2付小梅 2范崔生3
作者信息
- 1. 江西中医药大学药学院,南昌 330004
- 2. 江西中医药大学药学院,南昌 330004;范崔生全国名中医传承工作室,南昌 330004
- 3. 范崔生全国名中医传承工作室,南昌 330004
- 折叠
摘要
以7批菟丝子和8批大菟丝子商品药材为材料,通过体视显微镜观测菟丝子和大菟丝子整体及种脐形状大小、四面观,分别将菟丝子和大菟丝子药材种皮进行组织解离制片,利用光学显微镜观测种皮栅状细胞断面观特征,用扫描电镜观测种脐特征,对菟丝子和大菟丝子药材进行形态组织研究并进行分类比较.结果表明,菟丝子以饱满为主,多为类球形,长与宽1～2 mm;四面观中腹面观略呈圆形,背面观略呈椭圆形,左面观和右面观略呈类圆形;种脐多为扁圆形.大菟丝子类圆球形或三棱形,长与宽2～3 mm;腹面观和右面观类椭圆形,背面观类半扇形,左面观类扇形,种脐圆形.显微鉴别发现,菟丝子种皮栅状细胞断面观有2层,内列细胞径向长为外列的1～2倍,而大菟丝子只有1层,径向长30.62～41.27μm.扫描电镜观测菟丝子脐孔呈唇形翻起状,长137.58～217.00 μm,宽6.00～21.00μm;大寃丝子脐孔呈衣领状,长 464.52～792.51μm,宽 5.75～28.19 μm.
Abstract
7 batches of Cuscutae Semen and 8 batches of Cuscutae Japonicae Semen were used as mate-rials.Cuscutae Semen and Cuscutae Japonicae Semen were observed by stereopicroscope as well as the whole body shape and size of the seed umbilicus,the four-sided view;Cuscutae Semen Semen and Cuscutae Japonicae Semen seed coats were separated and prepared,optical microscope was used to observe the cross section features of seed coat grid cells;the characteristics of species umbilicus were observed by scanning electron microscopy,to study the morphological organization of Cuscutae Semen and Cuscutae Japonicae Semen and made a classification comparison.The results showed that the characters of Cuscutae Semen were mainly plump,of which were quasi-spherical,the length and width were about 1-2 mm;the central ventral view was slightly circular,the back view was slightly elliptical,the left view and the right view were slightly quasi-circular,the umbilicus were mostly oblate.Cuscutae Japonicae Semen was spherical or triangular,the length and width were about 2-3 mm;the ventral view and the right view were elliptical,the back view was semi-fan-shaped,and the left view was fan-shaped,the umbilical was round.Through the microscopical identification,it was observed that the section of Cuscutae Semen seed coat grating cells had 2 layers,and the radial length of the inner row was about 1-2 times that of the outer row,while Cuscutae Japonicae Semen only had 1 layer,the radial length was about 30.62-41.27 μm.The umbilical opening of Cuscutae Semen were labial upturned,137.58-217.00 μm in long and 6.00-21.00 μm in wide by scanning electron microscopy;The umbilical opening of Cuscutae Japonicae Semen were collared,the length was 464.52-792.51 μm,the width was 5.75-28.19 μm.
关键词
菟丝子/大菟丝子/性状鉴别/分类学/显微鉴别Key words
Cuscutae Semen/Cuscutae Japonicae Semen/trait identification/taxonomy/microscopic identification引用本文复制引用
基金项目
江西省中管局科技计划项目(2020B0384)
江西省卫计委科技计划项目(20175543)
江西中医药大学中药学一流学科建设科研项目(JXSYLXK-ZHYAO026)
全国中药特色技术传承人才培训项目(国中医药人教函[2018]204号)
范崔生全国名中医传承工作室(国中医药人教函[2018]119号)
第六批全国老中医药专家学术经验继承工作(国中医药人教发[2017]29号)
出版年
2023
国家科技期刊平台
NSTL
维普
万方数据