Morphological and Transcriptome Analysis on Response of Potentilla anserina to Salt-alkali Stress
To investigate the effects of different salt-alkali stress on the growth of Potentilla anserina and through transcriptome sequencing analysis,the epigenetic morphological regulation and molecular mechanisms of salt-alkali tolerance in Potentilla anserina were preliminarily explored.Soil cultivation was employed to subject Potentilla anserina to varying concentrations of NaCl and NaHCO3 stress.The responses of morphological traits,such as plant height and stem thickness,to different levels of salt-alkali stress were analysed.In addition,Illumina high-throughput sequencing technology was utilized for transcriptome sequencing to investigate the impact of salt-alkali stress on the transcrip-tional levels of Potentilla anserina.Results showed that under salt-alkali stress,Potentilla anserina exhibited higher tolerance to salt than to alkali.As stress concentrations increased,Potentilla anseri-na s agronomic traits displayed an initial increase followed by a decrease,overall showing a'low pro-motion,high inhibition'pattern.Malondialdehyde(MDA)content showed an initial increase followed by a decrease with increasing stress concentration,indicating significant membrane lipid peroxidation.When stress concentrations exceeded a certain threshold,both the antioxidant and osmotic regulation systems were unable to alleviate damage to the membrane system.Transcriptome sequencing genera-ted a total of 82.95 Gb of data,with clean data for each sample reaching 8.41 Gb or more,and a Q30 base percentage of 93.17％or higher.Through differential gene expression(DEGs)analysis,1 616,1 590 and 1 770 DEGs in the three comparison groups(ck-vs-NaCl,ck-vs-NaHCO3,NaCl-vs-NaH-CO3)were identified.GO enrichment analysis of DEGs revealed significant enrichment of terms relat-ed to salt response,flavonoid biosynthesis,nitrate reductase(NADPH),and more.KEGG analysis of DEGs indicated significant enrichment of pathways including phenylpropanoid biosynthesis,flavonoid biosynthesis,MAPK signaling,and plant hormone signal transduction.Furthermore,among the identified DEGs,members of the ERF,MYB,NAC,WRKY.MYB and bZIP transcrip-tion factor families were the most abundant.Potentilla anserina primarily adaptted to stress environ-ments by increasing stem thickness and reducing leaf area,exhibiting an overall'low promotion,high inhibition'trend with a certain threshold.Potentilla anserina responded to its salt-alkali environment by modulating membrane composition,redox enzyme activity,flavonoid biosynthesis,phenylpro-panoid biosynthesis,MAPK signaling,and plant hormone signal transduction,among other biologi-cal processes and metabolic pathways.
Potentilla anserinesaline-alkali stresstranscriptometranscriptome factorsmechanism of saline-alkali resistancedifferentially expressed gene
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维普
