摘要
为了解中波紫外线(UV-B)对糯玉米种子活力影响的分子机理,设置0 min(对照)、30 min、1 h、2 h、4 h和8 h共6个不同时长的UV-B辐照对糯玉米自交系N51种子进行处理,同时进行相关活力指标和转录组学分析.结果表明,糯玉米自交系N51各处理与对照及各处理间差异均不显著.在0 min、30min、1 h、2h、4h、8h范围内,发芽势、发芽指数和活力指数均呈先上升后下降的趋势,且在1 h处理下达到最高,综合各个活力指标表明,UV-B辐照处理1 h促进玉米种子萌发效果最显著.依据0 min、1 h、2 h等3个时长处理下,糯玉米种子活力指标呈先升后降的趋势,转录组测序分析共筛选出先上调再下调的差异表达基因5个,先下调后上调的差异表达基因869个.GO注释分类表明,UV-B处理主要通过影响类囊体、光合系统光合作用、光合膜、光合作用-天线蛋白、代谢途径、氮代谢途径等影响糯玉米自交系N51种子萌发;KEGG通路富集包括苯丙素类生物合成、类黄酮生物合成、淀粉和蔗糖代谢等途径.
Abstract
In order to understand the molecular mechanism of the effect of medium wave ultraviolet(UV-B)on the activity of waxy maize seeds,waxy maize inbred line N51 seeds were treated with UV-B irradiation at 0 min(control),30 min,1 hours,2 hours,4 hours and 8 hours,and the activity in-dexes and transcriptomics were analyzed.The results showed that there was no significant difference between waxy maize inbred line N51 and the control.In the range of 0 min,30 min,1 hours,2 hours,4 hours and 8 hours,the germination potential,germination index and vitality index all showed a trend of first increasing and then decreasing,and the treatment length reached the highest at 1 hours.According to various vitality indexes,UV-B irradiation at 1 hours had the most significant effect on promoting corn seed germination.According to 0 min(control),1 hours,2 hours 3 treatments,the seed vitality indexes of waxy maize showed a trend of first increasing and then decreasing.Transcrip-tome sequencing analysis screened out 5 differentially expressed genes that were first up-regulated and then down-regulated,and 869 differentially expressed genes that were first down-regulated and then up-regulated.GO annotation classification showed that UV-B treatment mainly affected the differential expression of germination-related phenotypes of waxy maize inbreeding line N51 seeds by affecting th-ylakoids,photosynthetic system light,cooperative use,photosynthetic membrane,photosynthetic antenna protein,metabolic pathway,nitrogen metabolic pathway and other metabolic pathways.The enrichment of KEGG pathway included phenylpropanoid biosynthesis,flavonoid biosynthesis,starch and sucrose metabolism.
基金项目
广东省农业农村厅农业科研类及技术推广示范类项目(粤财农[2022]189号)
广东省普通高等学校自然科学类重点领域专项(2023)(2023ZDZX4017)
广州市财政农业农村项目(22304496)
广东省阳江市省科技创新专项(2023)(SDZX2023024)
维普
万方数据