首页|利用小孢子培养技术创制红菜薹早熟DH系

利用小孢子培养技术创制红菜薹早熟DH系

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为获得早熟型的双单倍体(DH)植株,加快早熟型红菜薹育种进程,本研究以15个早熟型红菜薹品种为试材,进行小孢子培养,观察小孢子发育过程并对再生植株进行倍性鉴定.结果表明,当花瓣/花药(P/A)长度比值为0.6～0.8时,红菜薹小孢子72％～78％处于单核靠边期;共有6个基因型红菜薹出胚,其中出胚率最大的为gy21-55,平均出胚率为14.3胚/蕾;选取子叶形胚进行植株再生,成苗率在90％以上;利用流式细胞仪对再生植株进行倍性鉴定,平均自然加倍率达76.8％.gy21-40、gy21-55表现出早熟特性,与对照植株相比获得的DH植株可分别提早5 d和8 d抽薹.
Creation of Early-maturing DH Lines of Brassica campestris var.purpuraria through Microspore Culture Technique
In order to obtain early maturing double haploid(DH)plants and accelerate the breeding process of early maturing Brassica campestris var.purpuraria.This study used 15 early maturing Brassica cam pestris var.pur puraria varieties as test materials for microspore culture,observing the development process of microspores and identifying ploidy of regenerated plants.The results indicated that when the petal/anther(P/A)length ratio was between 0.6 and 0.8,approximately 72％to 78％of the microspores of red amaranth were in the uninucleate stage at the periphery;A total of 6 geno-types of red amaranth were able to produce embryos,with gy21-55 exhibiting the highest embryogen-esis rate,the average embryogenesis rate was 14.3 embryos per bud;The cotyledonary embryos were selected for plant regeneration,and the plantlet conversion rate exceeded 90％;Flow cytometry was utilized to determine the ploidy level of the regenerated plants,with an average natural doubling rate of 76.8％.Gy21-40 and Gy21-55 exhibited early maturity traits,and the DH plants obtained from them showed a respective advancement of 5 days and 8 days in bolting compared to the control plants.

Brassica cam pestris var.pur purariaisolated microspore cultureplant regenerationearly-maturing DH

赵丽芬、邓英、付文苑、王青青、杨巍

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贵州大学农学院,贵阳 550025

贵州省农业科学院园艺研究所,贵阳 550006

红菜薹 游离小孢子培养 植株再生 早熟型DH系

贵州省科技计划贵州省科技计划贵州省科技计划国家自然科学基金贵阳市蔬菜种质资源研究中心建设项目贵州省蔬菜产业技术体系建设项目

黔科合支撑[2020]1Y090号黔科合支撑[2022]一般黔科合服企[2022]00531960595筑科合同[2021]5-1号GZCYTX2023-01

2024

10.16590/j.cnki.1001-4705.2024.01.137

种子
贵州省种子管理站 贵州省种子学会 中国种子协会

种子

CSTPCD北大核心
影响因子：0.502
ISSN：1001-4705
年,卷(期)：2024.43(1)
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