Research on the Response of Rice Glutamate Receptor to Low Temperature Stress
Rice is susceptible to damage under low temperature stress.Glutamate receptors(GLRs),known as cold sensors in nematodes,mice,and human neurons,have not been fully understood for their low-tem-perature effects in plants.In order to investigate the low temperature response function of rice GLRs in depth,the study on the phenotype,physiological indicators,and gene expression patterns of rice materials under low temperature stress after treatment with glutamate receptor agonists were conducted.The results of the study showed that treatment with 10 mmol/L concentration of glutamate receptor agonist enhanced the cold toler-ance of rice seedlings.Further physiological and molecular biology experiments revealed that rice seedlings im-proved their cold tolerance under low temperature conditions by regulating the expression of physiological indicators such as superoxide dismutase,catalase,malondialdehyde,and chlorophyll content.Additionally,using CRISPR/Cas9 gene editing technology,knockout mutants of the OsGLR6.5 and OsGLR6.9 genes were sucessfully constructed,and it was found that the OsGLR6.5 gene could play an important regulatory role in rice low temperature stress.These findings were of significant importance for a deeper understanding of the adaptive mechanisms of rice to low temperature environments.
万方数据
维普
