Microscopic observation and high-performance liquid chromatography were used to analyze the biological,microscopic,and chemical properties of seeds from Daxinganling and Shanxi Province,and tran-scriptome sequencing was used to perform the sequencing study on substance formation difference mechanisms of germplasms,in order to provide an essential reference for germplasm screening and plant variety selection.The results showed that the biological characteristics like seed moisture,water absorption and viability of the Trollius chinensis Bunge seeds from Daxinganling were generally better than those from Shanxi Province.The HPLC method was used to determine the contents of orientin and vitexin in the Daxinganling.The contents of orientin and vitexin in Daxinganling were 0.014 3-0.014 6 mg/g and 0.840-0.868 mg/g,respectively.Tran-scriptome sequencing was performed on the seeds of the two regions,and a total of 32 959 Unigenes were assembled.3 165 genes with differential expression were found in total,and S-adenosyl-L-methionine:3'-hy-droxy-N-methylcoclaurine 4'-O-methyltransferase,aldehyde dehydrogenase(NAD(P)(+))ald5,chalcone synthase 2,plant peroxidase,and other key enzyme genes could be closely related to phenylpropanoid biosyn-thesis,isoquinoline alkaloid biosynthesis,and flavonoid biosynthesis.
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