首页|寒地水稻孕穗期低温胁迫响应的转录组分析

寒地水稻孕穗期低温胁迫响应的转录组分析

扫码查看
水稻是世界重要的口粮作物,其整个生育周期都会受到低温冷害的威胁.为探究寒地水稻孕穗期低温胁迫应答基因的发掘及其低温应答调控机制,本研究对孕穗期低温下寒地水稻孕穗期耐冷品种龙粳25(LG25)和不耐冷品种龙粳11(LG11)的幼穗颖花展开转录组测序分析.结果表明,龙粳11中共筛选得到11 269个差异表达基因(DEGs),龙粳25中仅有1148个DEGs.GO富集分析表明,龙粳11中差异基因多显著富集在光合作用相关的GO条目中,龙粳25多富集在低温、热响应及花粉发育相关的GO条目中.低温胁迫下,二者共同显著富集到低温响应及花粉发育GO条目.KEGG富集分析表明,龙粳11中差异基因多显著富集在光合有机物中的碳固定、光合作用等KEGG通路中,龙粳25多显著富集在淀粉蔗糖代谢、氨基糖和核糖代谢等KEGG通路中.
Transcriptome Analysis of Rice Response to Low Temperature Stress at Booting Stage in Cold Region
Rice is an important food crop in the world,and the whole growth cycle will be threatened by low temperature.In order to explore the discovery of low temperature stress response genes and the regulation mechanism of low temperature response,transcriptome sequencing was performed on young spikelets of cold tolerant variety Longgeng25(LG25)and cold sensitive variety Longgeng11(LG11)under cold stress at booting stage in cold region.The results showed that a total of 11 269 differentially expressed genes(DEGs)were obtained from LG11,while only 1 148 DEGs were obtained from LG25.The GO enrichment analysis showed that the differential genes in LG11 were mostly significantly enriched in the GO terms related to photosynthesis,while LG25 were mostly enriched in the GO terms related to cold response,heat response and pollen development.Under cold stress,both of them significantly enriched to cold response and pollen development.KEGG enrich-ment analysis showed that most of the DEGs in LG11 were significantly enriched in KEGG pathways such as carbon fixation in photosynthetic organic matter and photosynthesis.LG25 was significantly enriched in KEGG pathways such as starch sucrose metabolism,amino sugar metabolism and ribose metabolism.

cold region ricebooting stagecold stress responsetranscriptome sequencing

郭震华、蔡丽君、刘传雪、王瑞英、关世武、黄晓群、郭俊祥、张兰民、潘国君

展开 >

黑龙江省农业科学院水稻研究所,黑龙江佳木斯 154026

黑龙江省农业科学院佳木斯分院,黑龙江佳木斯 154007

寒地水稻 孕穗期 低温胁迫响应 转录组测序

黑龙江省省属科研业务费项目黑龙江省农科院农业科技基础创新项目杰出青年项目黑龙江省博士后面上资助项目黑龙江省自然科学基金优秀青年基金

CZKYF2021-2-C002CX23JQ02LBH-Z21027YQ2021C033

2024

种子
贵州省种子管理站 贵州省种子学会 中国种子协会

种子

CSTPCD北大核心
影响因子:0.502
ISSN:1001-4705
年,卷(期):2024.43(2)
  • 28