Analysis of Male Sterility Variation of BBLa Overexpressed Tobacco Based on Re-sequencing Technology
BBLa gene encodes Berberine bridge enzyme-like,and this enzyme is involved in the last catalytic step of nicotine biosynthesis.In the previous study,It was found that stamen abortion phenotype appeared in T0 generation plants of BBLa overexpressed tobacco.In order to further under-stand the genetic mechanism of this male sterility phenotype,based on genome-wide re-sequencing technology,this study used BBLa overexpressed tobacco male sterility phenotype OE1 and wild type(WT)tobacco as materials to mark and annotate three variation types:single nucleotide polymor-phism(SNP),insertion-deletion(InDel)and chromosome structural variation(SV),with an average sequencing depth over 14× and an average coverage rate over 95％.The results showed that 7 505 620 SNP,628 034 InDel and 79 576 SV were detected in two samples.Through the comparison SNP differences between the two samples,174 candidate genes were finally obtained.
万方数据
维普
