In this study,the Illumina HiSeqTM 6000 platform was used to analyze the transcriptome sequencing of Pinus massoniana seeds before and after cryopreservation.A total of 51.79 Gb filter data were obtained.A total of 2 122 differentially expressed genes were identified before and after cryopreservation,of which 823 were up-regulated and 1 299 were down-regulated.GO enrichment analysis showed that 163 differentially expressed genes were significantly enriched in 33 GO terms,and KEGG enrichment analysis showed that 169 differentially expressed genes were significantly enriched to 79 pathways.According to the results of KO and KEGG,19 differentially expressed genes were selected from the plant hormone signal transduction pathway,plant MAPK signal pathway,starch and sucrose metabolism pathway,and transcription factor pathway.
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