In order to select excellent Epimedium germplasm to ensure the stability of its quality,the agronomic characters,mineral elements,yield and quality of 15 Epimedium germplasm were deter-mined and comprehensively evaluated.The results showed that the plant height,branch number,leaf number,leaf area,leaf width,leaf length,bud head number,fibrous root,rhizome and leaf yield of Epimedium sagittatum germplasm collected from Congjiang County,Guizhou Province were more better than others,which was 27.40 cm,28.44 branches,67.33 pieces,1 127.09 cm2/plant,6.23 cm,9.64 cm,12.78 pieces,3.74g/plant,43.49 g/plant and 13.39 g/plant,respectively.The content of N,P and K in Epimedium sagittatum from Congjiang,Guizhou was 17.51 g/kg,1.57 g/kg and 9.40 g/kg,respectively.The content of total flavonoids and total flavonol glycosides of Epimedium sagit-tatum from Congjiang,Guizhou was the highest,with 12.68%and 10.79%,respectively.Combined with the principal component analysis,membership function analysis and comprehensive evaluation of agronomic characters,mineral elements and medicinal material quality,Epimedium sagittatum from Congjiang,Guizhou had the highest score in plant height,leaf area,leaf width,leaf length,number of buds,dry weight of fibrous roots,rhizomes,stems,leaves,total flavonoids and total flavonol glycosides.