种子2024,Vol.43Issue(2) :88-94.DOI:10.16590/j.cnki.1001-4705.2024.02.088

镉胁迫对紫丁香种子萌发及幼苗生长的影响

Effects of Cadmium Stress on Seed Germination and Seedling Growth of Syringa oblata L.

孙全航 康璠 何淼 王若彤 杨雪婷 郭亚男 姜迦媛 高雪岚 李强
种子2024,Vol.43Issue(2) :88-94.DOI:10.16590/j.cnki.1001-4705.2024.02.088

镉胁迫对紫丁香种子萌发及幼苗生长的影响

Effects of Cadmium Stress on Seed Germination and Seedling Growth of Syringa oblata L.

孙全航 1康璠 1何淼 1王若彤 1杨雪婷 1郭亚男 1姜迦媛 1高雪岚 1李强1
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作者信息

  • 1. 东北林业大学园林学院,哈尔滨 150040
  • 折叠

摘要

本研究以紫丁香种子和幼苗为研究对象,分析了不同浓度(0、5 mg/L、10 mg/L、20 mg/L、30 mg/L、40 mg/L)镉胁迫对种子萌发、幼苗生长生理特性及镉积累特性的影响.结果表明,随着镉胁迫浓度升高,种子各项发芽指标呈先升高后降低的趋势,在胁迫浓度为5 mg/L时发芽率最高.异常发芽率在胁迫浓度大于10 mg/L时显著升高(p<0.05).由回归分析可知,镉胁迫对种子萌发的半致死浓度为29.95 mg/L,极限浓度为50.41 mg/L.镉胁迫对胚根生长的抑制作用要高于胚芽.随着镉胁迫浓度升高,幼苗地上、地下部干重及根冠比呈降低趋势,根系活力、抗氧化酶活性及渗透调节物质含量呈先升后降的趋势,并在胁迫浓度为10 mg/L时最高,MDA含量、质膜透性呈升高趋势,但在胁迫浓度小于10 mg/L时较对照无显著差异;镉转运系数在胁迫浓度为10 mg/L时最高,为0.54,积累量为1.25 mg/g.说明在镉胁迫浓度低于10 mg/L时,紫丁香幼苗能通过自身调节来维持正常生长,对镉胁迫具有一定耐性.

Abstract

In this paper,Syringa oblata L.seeds and seedlings were used as research object,and the effects of different concentrations of cadmium(0,5 mg/L,10 mg/L,20 mg/L,30 mg/L,40 mg/L)stress on seed germination,seedling growth physiological characteristics and cadmium accumulation characteristics were analyzed.The results showed that with the increase of cadmium stress concentra-tion,the germination index of seeds increased first and then decreased,and the germination rate was the highest when the stress concentration was 5 mg/L.Abnormal germination rate significantly increased when stress concentration was greater than 10 mg/L(p<0.05).According to the regression analysis,the semi-lethal concentration of cadmium stress on seed germination was 29.95 mg/L,and the limit concentration was 50.41 mg/L.The inhibitory effect of cadmium stress on radicle growth was higher than that of germ.With the increase of cadmium stress concentration,the above-ground and underground dry weight and root-shoot ratio of seedlings showed a decreasing trend,and the root activity,antioxidant enzyme activity and osmotic regulatory substance contents increased first and then decreased,reaching the highest at the stress concentration of 10 mg/L.The MDA content and plasma membrane permeability showed an increasing trend,but there was no significant difference compared with the control at the stress concentration of less than 10 mg/L.The highest Cd transfer coefficient was 0.54 when the stress concentration was 10 mg/L,and the accumulation amount was 1.25 mg/g.These results indicated that when the cadmium stress concentration was lower than 10 mg/L,Syringa oblata L.seedlings could maintain normal growth by adjusting their physiological activity and had a certain tolerance to cadmium stress.

关键词

镉胁迫/紫丁香/种子萌发/生理响应/积累转运

Key words

cadmium stress/Syringa oblata L./seed germination/physiological response/accumulation and transport

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基金项目

中央高校基本科研业务费专项(257214BA21)

国家重点研发计划子课题(2019YFD100150)

东北林业大学大学生创新训练计划(2021)(202110225393)

出版年

2024
种子
贵州省种子管理站 贵州省种子学会 中国种子协会

种子

CSTPCD北大核心
影响因子:0.502
ISSN:1001-4705
参考文献量30
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